仙人掌质量标准提升研究
|
王胜军，张洪铭，陈心智，徐雅娟，解生旭
|
吉林省中医药科学院，吉林长春，130021
|
摘要：目的 建立仙人掌中水仙苷含量测定方法，完善其质量控制标准。方法 采用高效液相色谱法（HPLC）测定仙人掌中水仙苷含量。色谱条件为：采用C18色谱柱，以乙腈 -0.1%甲酸水溶液（18:82，V/V）为流动相，流速1.0mL/min，检测波长254nm，柱温30℃，进样量10μL。结果 水仙苷在3.54 ~ 56.64μg范围内线性关系良好（R2=0.9997）。精密度、稳定性及重复性试验的RSD均低于2.0%，平均加样回收率为98.79%，RSD为1.15%（n=6）。通过3批仙人掌样品中水仙苷的含量测定，初步拟定仙人掌含仙水苷（C28H32O16）不得少于0.0105%。结论 所建立的HPLC方法简便、稳定、准确，可为仙人掌的质量评价与质量控制提供参考。
关健词：仙人掌；质量标准；高效液相色谱法；水仙苷 |
Study on the Improvement of Quality Standards of Opuntia stricta(Haw.) Haw. var. dillenii (Ker Gawl.) Benson
|
Shengjun Wang，Hongming Zhang,Xinzhi Chen,Yajuan Xu,Shengxu Xie
Jilin Provincial Academy of Traditional Chinese Medicine,Changchun Jilin 130021,China
|
Abstract: Objective To establish a quantitative method for the determination of narcissoside in Opuntia stricta (Haw.) Haw. var. dillenii (Ker Gawl.) Benson and to improve its quality control standard. Methods High-performance liquid chromatography (HPLC) was used to determine the content of narcissoside. The chromatographic conditions were as follows: a C18 column was employed with acetonitrile-0.1% formic acid aqueous solution (18:82, v/v) as the mobile phase at a fl ow rate of 1.0 mL/min. The detection wavelength was 254 nm, the column temperature was maintained at 30 °C, and the injection volume was 10 μL. Results Good linearity was observed over the range of 3.54–56.64 μg (R2 = 0.9997). The relative standard deviations (RSDs) of precision, stability, and repeatability were all less than 2.0%. The average recovery was 98.79% with an RSD of 1.15% (n = 6). Based on the determination of three batches of samples, the content of narcissoside was preliminarily specifi ed as not less than 0.0105% in Opuntia stricta (Haw.) Haw. var. dillenii (Ker Gawl.) Benson. Conclusion The established HPLC method is simple, stable, and accurate, providing a reliable reference for the quality evaluation and quality control of Opuntia stricta var. dillenii.
Keywords : Opuntia stricta var. dillenii; Quality standard; High-performance liquid chromatography; Narcissoside
参考文献
[1] 回瑞华 , 侯冬岩 , 李铁纯 , 等 . 仙人掌中黄酮类化合物的光谱分析及抗氧化性能测定 [J]. 鞍山师范学院学报,2010,12(6):47-50.
[2] 林爱琴,何爱明.仙人掌多糖对糖尿病小白鼠降糖作用的研究[J].重庆工商大学学报:自然科学版,2015,32(10):33-37.
[3] 戴小华 , 李春红 , 阿依姑丽·艾合买提 , 等 . 仙人掌总黄酮对小鼠抗急性炎症作用的研究 [J]. 中国农学通报,2013,29(36):400-403.
[4] 王丹,王文君.水仙苷的药理活性[J].辽宁大学学报(自然科学版),2024,51(2):113-120.
[5] 陈莹,黄锦芳,林娜,等.HPLC法同时测定金线莲中6种成分[J].中成药,2018,40(10):2222-2227.
[6] 布比阿加尔·哈依拉提 , 秦亚迪 , 王宇 , 等 .HPLC法同时测定金雀花中 6 种黄酮类成分 [J]. 中 成药,2023,45(2):470-475.
[7] 张嘉嘉,陈涛,鲍文,等.HPLC法测定福建金线莲中水仙苷的含量[J].福建中医药,2021,52(5):58-60.
[8] 何佳佳,张文琦,楼永军.复方芦丁片中有关物质的测定[J].中国药事,2025,39(11):1282-1297.
[9] 胡剑虹 , 周海琴 , 曹杰楠 , 等 . 金毛耳草药材 UPLC 指纹图谱建立及含量测定研究 [J]. 国际中医中药杂志,2025,47(2):229-236.
[10] 陈昭.基于多指标含量测定的舒脉方质量标准研究[C]//中国中西医结合学会.2017年第五次世界中西医结合大会论文摘要集(上册).2017:639.
[11] 朱东霞,谭梅英,詹利之.HPLC法同时测定通脉方中三种活性成分[J].海峡药学,2017,29(7):66-68. |
|
|
 |
论文收录证明 / 文献检索报告
Document Retrieval Certificate / Proof of Publication Indexing
作者贡献声明 / 贡献确认书
Author Contribution Statement / Certificate of Authorship Contribution
同行评审报告 / 评审意见
Peer Review Report / Peer Review Comments
利益冲突
Conflict of Interest
作者声明不存在任何利益冲突。
The author declares no conflict of interest.
|
|
