粘委陵菜药材质量标准的研究
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王胜军，张洪铭，陈心智，徐雅娟，解生旭
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吉林省中医药科学院，吉林长春，130021
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摘要：目的 建立粘委陵菜药材质量标准，为其质量控制与规范应用提供依据。方法 收集10批粘委陵菜药材，采用性状鉴别、显微鉴别及粉末显微鉴别方法，对药材性状、根横切面组织构造及粉末特征进行研究；以儿茶素对照品和粘委陵菜对照药材为对照，建立薄层色谱鉴别方法；参照《中国药典》四部通则，对杂质、水分、总灰分及酸不溶性灰分进行测定。结果 对粘委陵菜药材性状、根横切面组织特征及粉末显微特征进行了系统描述；薄层色谱鉴别斑点清晰、分离度良好、专属性强。10批粘委陵菜药材杂质含量为0.7% ~1.3%，平均值为0.99%；水分含量为9.3% ~10.7%，平均值为10.09%；总灰分含量为8.2% ~ 10.7%，平均值为9.56%；酸不溶性灰分含量为0.70% ~ 1.54%，平均值为0.99%。结论 本研究建立的粘委陵菜药材质量标准方法稳定、可行，可为其质量评价、质量控制及标准制定提供参考依据。
关健词：粘委陵菜；质量标准；性状鉴别；显微鉴别；薄层色谱 |
Study on the Quality Standard of Potentilla longifolia Willd. ex Schlecht. Medicinal Materials
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Shengjun Wang，Hongming Zhang,Xinzhi Chen,Yajuan Xu,Shengxu Xie
Jilin Provincial Academy of Traditional Chinese Medicine,Changchun Jilin 130021,China
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Abstract: Objective To establish a quality standard for Potentilla longifolia medicinal materials and provide a basis for their quality control and standardized application. Methods Ten batches of Potentilla longifolia medicinal materials were collected. Macroscopic identifi cation, microscopic identification, and powder microscopic identification were employed to investigate the macroscopic characteristics, root transverse section structures, and powder features of the medicinal materials. Thin-layer chromatography (TLC) identification was established using catechin reference substance and Potentilla longifolia reference medicinal material as references. According to the general chapters of the Chinese Pharmacopoeia, tests for impurities, moisture, total ash, and acid-insoluble ash were conducted. Results The macroscopic characteristics, root transverse section features, and powder microscopic characteristics of Potentilla longifolia were systematically described. The TLC chromatograms showed clear spots, good separation, and strong specifi city. Among the 10 batches of samples, impurity content ranged from 0.7% to 1.3%, with an average of 0.99%; moisture content ranged from 9.3% to 10.7%, with an average of 10.09%; total ash content ranged from 8.2% to 10.7%, with an average of 9.56%; acid-insoluble ash content ranged from 0.70% to 1.54%, with an average of 0.99%. Conclusion The quality standard established in this study is stable and feasible, and can provide a reference for the quality evaluation, quality control, and standard development of Potentilla longifolia medicinal materials.
Keywords : Potentilla longifolia Willd. ex Schlecht.; Quality standard;Macroscopic identifi cation;Microscopic identifi cation; Thinlayer chromatography
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